Additional file 1 of California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting

Additional file 1: Supplementary Table S1. GenBank accessions of full-length RNA1 and RNA2 genomes of different TRV isolates. Supplementary Table S2. List of primers used. Supplementary Table S3. Effect of different growth temperatures on PDS gene silencing in N. attenuata induced by TRV California VIGS vectors (pTRV-RNA1/pTRV-RNA2:PDS). Supplementary Table S4. Monthly temperatures in Santa Barbara, CA in 2009. Supplementary Table S5. Monthly average temperatures in Scotland 1971–2000. Supplementary file 2: Supplementary Figures. Supplementary figure S1. Disease symptoms in host plant species mechanically infected with TRV California. Supplementary figure S2. Detection of the TRV infection in host plants. Supplementary Figure S3. N. attenuata plants inoculated with the TRV California vector system, grown at different temperatures. Supplementary figure S4. Systemic silencing of the PDS gene in N. attenuata induced with the TRV California and PpK20 vectors after growth at 28°C and 30°C (sap inoculated). Supplementary figure S5. Swapping of RNA1 and RNA2 vectors of California and PpK20 isolates. Supplementary figure S6. ClustalW analysis of the RNA dependent RNA polymerase (RdR) proteins from the TRV California and TRV PpK20 isolates. Supplementary figure S7. ClustalW analysis of the 16 kDa Suppressor proteins (A), Movement proteins (B) and Coat proteins (C) from the TRV California and TRV PpK20 isolates. Supplementary file 3: Original gel images of Fig. 1D, E, S2A and S2B with legends. Supplementary file 4: Original gel image file (JPEG format) Fig. 1D. Supplementary file 5: Original gel image file (JPEG format) Fig. 1E. Supplementary file 6: Original gel image file (JPEG format) Fig. S2A. Supplementary file 7: Original gel image file (JPEG format) Fig. S2B.附加文件1：补充表S1。不同烟草脆裂病毒（TRV）分离物的全长RNA1和RNA2基因组的基因银行（GenBank）登录号。补充表S2：所用引物列表。补充表S3：TRV加州株病毒诱导基因沉默（VIGS）载体（pTRV-RNA1/pTRV-RNA2:PDS）诱导本氏烟草（N. attenuata）中PDS基因沉默的不同培养温度效应。补充表S4：2009年加利福尼亚州圣巴巴拉的月度气温。补充表S5：1971-2000年苏格兰月度平均气温。 附加文件2：补充图。补充图S1：机械接种TRV加州株的寄主植物的病害症状。补充图S2：寄主植物中TRV侵染的检测。补充图S3：在不同温度下培养的、经TRV加州株载体系统接种的本氏烟草植株。补充图S4：在28℃和30℃下培养后，经TRV加州株和PpK20载体诱导的本氏烟草中PDS基因的系统性沉默（汁液接种法）。补充图S5：加州株与PpK20分离物的RNA1和RNA2载体互换实验。补充图S6：TRV加州株与TRV PpK20分离物的RNA依赖RNA聚合酶（RdR）蛋白的ClustalW分析。补充图S7：TRV加州株与TRV PpK20分离物的16 kDa抑制蛋白（A）、运动蛋白（B）和外壳蛋白（C）的ClustalW分析。 附加文件3：带有图例的图1D、E以及图S2A、S2B的原始凝胶电泳图像。 附加文件4：图1D的原始凝胶图像文件（JPEG格式）。 附加文件5：图1E的原始凝胶图像文件（JPEG格式）。 附加文件6：图S2A的原始凝胶图像文件（JPEG格式）。 附加文件7：图S2B的原始凝胶图像文件（JPEG格式）。