Genome-wide DNA methylation analysis of psoriatic and normal skin tissues. Homo sapiens

Genome wide DNA methylation profiling of psoriatic (PP), paired uninvolved psoriatic (PN) and normal skin tissues (NN). The Illumina Infinium 450k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 485,000 CpGs in 135 PP, 41PN and 62 NN tissues which were obtained from those who underwent skin biospsies. We not only revealed a genome-wide methylation level pattern for psoriasis, but also identified a strong association between the skin-specific DNAm of 9 DMS and psoriasis. Overall design: Bisulphite converted DNA from the 217 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip v1.2

本数据集针对银屑病皮损皮肤（psoriatic plaque, PP）、配对银屑病未受累皮肤（paired uninvolved psoriatic, PN）及正常皮肤组织（normal skin, NN）开展全基因组DNA甲基化谱分析。本研究采用Illumina Infinium 450k人类DNA甲基化微珠芯片v1.2（Illumina Infinium 450k Human DNA methylation Beadchip v1.2），对135份PP样本、41份PN样本及62份NN样本进行检测，覆盖约485,000个CpG位点的DNA甲基化谱信息，所有样本均取自接受皮肤活检的受试者。本研究不仅揭示了银屑病的全基因组甲基化水平模式，还鉴定出9个差异甲基化位点（Differentially Methylated Sites, DMS）的皮肤特异性DNA甲基化与银屑病存在显著关联。实验设计：将217份样本的亚硫酸氢盐转化DNA（Bisulphite converted DNA）与Illumina Infinium 450k人类DNA甲基化微珠芯片v1.2进行杂交。