Anovab'b'.

The aim of this study was to investigate the effect of zinc sulphate on the activities of different enzymes and metabolites of Pholiota adiposa. In the experiment, we used the conventional enzyme activity assay to determine the changes of six indicators, including protein content, laccase activity, cellulase activity, amylase activity and polyphenol oxidase activity, under different concentrations of zinc sulphate treatment. The results showed that the activities of amylase, laccase, cellulase and peroxidase were Zn2+(200)>Zn2+(0)>Zn2+(400)>Zn2+(800).The activities of catalase and superoxide dismutase were Zn2+(200)>Zn2+(400)>Zn2+(800), and zinc sulfate could significantly affect the activity of polylipic squamase in a dose-dependent manner. Further correlation analysis showed that all six enzyme activities were significantly correlated with each other (PPholiota adiposa.本研究旨在探究硫酸锌（zinc sulphate）对肥鳞菇（Pholiota adiposa）不同酶活性及代谢物的影响。实验中，我们采用常规酶活性测定方法，检测不同浓度硫酸锌处理下六项指标的变化，包括蛋白质含量、漆酶（laccase）活性、纤维素酶（cellulase）活性、淀粉酶（amylase）活性及多酚氧化酶（polyphenol oxidase）活性。结果显示，淀粉酶、漆酶、纤维素酶与过氧化物酶（peroxidase）的活性均遵循Zn²⁺(200)＞Zn²⁺(0)＞Zn²⁺(400)＞Zn²⁺(800)的变化趋势。过氧化氢酶（catalase）与超氧化物歧化酶（superoxide dismutase）的活性则遵循Zn²⁺(200)＞Zn²⁺(400)＞Zn²⁺(800)的变化规律，且硫酸锌可通过剂量依赖性方式显著影响多脂鳞伞酶（polylipic squamase）的活性。进一步相关性分析表明，六项酶活性彼此间均存在显著相关性（PPholiota adiposa.）