Transcriptome Analysis of TubA Effects on Oocytes Development in Mice

TubA is a specific inhibitor of HDAC6, it could regulate oocytes maturation by regulating acetylation of a-tubulin. However, weather it effects maternal mRNA expression remains unkown. Therefore, in this study,we got in vitro maturated MII oocytes in control and 0.1µg/mL treated .Our study suggests that TubA mainly downregulated cell cycle and DNA methylation related genes. Overall design: single cell mRNA profiles of control and TubA treated MII oocytes were generated by deep sequencing, 5 samples for each group, using Illumina HiSeq 2000

TubA是组蛋白去乙酰化酶6（HDAC6）的特异性抑制剂，可通过调控α-微管蛋白（α-tubulin）的乙酰化水平调节卵母细胞成熟。但其是否影响母源mRNA的表达仍未明确。因此本研究收集了体外成熟的对照组与0.1μg/mL TubA处理组的MII期卵母细胞。本研究结果显示，TubA主要下调了细胞周期与DNA甲基化相关基因。整体实验设计：采用Illumina HiSeq 2000测序平台，对两组MII期卵母细胞进行单细胞mRNA表达谱深度测序，每组设置5个生物学重复样本。