Up-regulated miRNAs with predicted YY1 binding sites on promoter.

Genome-wide computational prediction reveals the prevalence of YY1 binding sites on promoters of miRNAs. C2C12 myoblasts were grown in growth medium (GM) or differentiated in differentiation medium (DM) for 3 days. Total RNAs were extracted and subjected to expression profiling using a microarray platform. 77 miRNAs were found to be up-regulated in DM compared to GM with a fold change (DM/GM) more than 2. These miRNAs were run through the YY1 binding site search pipeline. A total of 30 miRNAs were found to contain at least one YY1 binding site on their promoters.全基因组范围内的计算预测揭示了微小RNA（microRNA，miRNA）启动子上YY1结合位点的广泛分布。将C2C12成肌细胞置于生长培养基（growth medium，GM）中培养，或在分化培养基（differentiation medium，DM）中诱导分化3天；随后提取总RNA，采用微阵列（microarray）平台开展表达谱分析。相较于GM组，DM组中共鉴定出77个miRNA，其表达量显著上调，倍数变化（DM/GM）大于2。将上述77个miRNA提交至YY1结合位点搜索流程进行分析，最终共有30个miRNA的启动子区域含有至少一个YY1结合位点。