MeCP2 regulates genome-wide gene expression through recognition of H3K27me3 [ChIP, MNase]. MeCP2 regulates genome-wide gene expression through recognition of H3K27me3 [ChIP, MNase]

MeCP2 plays a multifaceted role in gene expression regulation and chromatin organization. Interaction between MeCP2 and methylated DNA to regulate gene expression is well established. However, the widespread MeCP2 distribution suggests its additional interactions with chromatin. Here we show, by both biochemical and ChIP-seq analyses, that MeCP2 directly binds to nucleosome subunit proteins and is recruited to distinct chromatin regions where H3K27me3 is enriched. We further observed that the impact of MeCP2 on transcriptional changes is correlated with histone post-translational modification patterns. Our findings indicate that MeCP2 can be recruited to genomic loci via indirect binding and that interaction between MeCP2 and histone proteins plays a significant role in gene expression regulation. Overall design: Examination of two epigenetic markers from the olfactory epithelium in two biological samples. This dataset is created to supplement the MeCP2 ChIP-seq and MNase-seq datasets (GSE71126) generated by Rube HT. Nature Communications 2016.

甲基化CpG结合蛋白2（MeCP2）在基因表达调控与染色质组织过程中发挥多方面作用。MeCP2与甲基化DNA的相互作用可调控基因表达，该结论已得到广泛证实。然而，MeCP2在基因组中的广泛分布提示，其可与染色质产生额外的相互作用。本研究通过生化分析与染色质免疫共沉淀测序（ChIP-seq）实验证实，MeCP2可直接结合核小体亚基蛋白，并被招募至富集组蛋白H3赖氨酸27三甲基化（H3K27me3）的特定染色质区域。我们进一步发现，MeCP2对转录水平变化的影响与组蛋白翻译后修饰模式密切相关。本研究结果表明，MeCP2可通过间接结合方式被招募至基因组位点，且MeCP2与组蛋白的相互作用在基因表达调控中发挥关键作用。

总体实验设计：对两份生物学样本的嗅觉上皮组织开展两种表观遗传标记物检测。本数据集用于补充Rube HT于2016年发表于《自然·通讯（Nature Communications）》的MeCP2染色质免疫共沉淀测序（ChIP-seq）与微球菌核酸酶测序（MNase-seq）数据集（登录号：GSE71126）。