Estimating relatedness and inbreeding using molecular markers and pedigrees: the effect of demographic history

Estimates of inbreeding and relatedness are commonly calculated using molecular markers, although the accuracy of such estimates has been questioned. As a further complication, in many situations, such estimates are required in populations with reduced genetic diversity, which is likely to affect their accuracy. We investigated the correlation between microsatellite- and pedigree-based coefficients of inbreeding and relatedness in laboratory populations of Drosophila melanogaster that had passed through bottlenecks to manipulate their genetic diversity. We also used simulations to predict expected correlations between marker- and pedigree-based estimates and to investigate the influence of linkage between loci and null alleles. Our empirical data showed lower correlations between marker- and pedigree-based estimates in our control (nonbottleneck) population than were predicted by our simulations or those found in similar studies. Correlations were weaker in bottleneck populations, confi...

近交与亲缘关系的估算值通常借助分子标记(molecular markers)进行计算，尽管此类估算的准确性曾饱受质疑。更为棘手的是，在诸多研究场景中，这类估算需应用于遗传多样性降低的种群，而这一情况很可能对估算准确性造成负面影响。我们以经瓶颈效应(bottleneck)处理以调控遗传多样性水平的实验室黑腹果蝇(Drosophila melanogaster)种群为研究对象，探究了基于微卫星标记(microsatellite)与基于系谱(pedigree)的近交系数、亲缘关系系数之间的相关性。此外，我们还通过模拟实验预测了标记推导与系谱推导的估算值间的理论相关性，并探究了位点间连锁(linkage)与无效等位基因(null alleles)的影响。我们的实测数据显示，对照组（非瓶颈种群）中标记推导与系谱推导的估算值间的相关性，低于本研究模拟实验的预测结果，也低于同类研究中观测到的相关性水平。瓶颈种群中的相关性则更弱，原文此处内容未完整呈现