Expression profile of mRNAs in PBMCs infected with CSF vaccine virus and Monocyte derived macrophages infected with virulent CSF virus of crossbred and indigenous pigs

Using high-throughput technology, we aim to identify the expression of mRNAs for understanding the comparative pathogenesis of CSF in PBMCs and Macrophages of indigenous verses crossbred pigs. Overall design: PBMCs were collected after 7 and 21 days post vaccination of CSF vaccine virus and 60 hours post infection with virulant CSF virus in MDMs of crossbred and indigenous pigs to identify the global gene expression

本研究采用高通量技术，旨在鉴定信使RNA（messenger RNA, mRNA）的表达水平，以解析本土猪与杂交猪的外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）及巨噬细胞（Macrophages）中猪瘟（Classical Swine Fever, CSF）的比较发病机制。整体实验设计：分别于接种猪瘟疫苗病毒后第7天、第21天采集杂交猪与本土猪的外周血单个核细胞；同时在强毒猪瘟病毒感染杂交猪与本土猪的单核细胞衍生巨噬细胞（monocyte-derived macrophages, MDMs）60小时后采集样本，以此鉴定全基因组的基因表达情况。