Intergenerational Lizard Lounges do not Explain Variation in the Gut Microbiomes of Green Iguanas

These samples represent the hindgut swabbings of 76 Green Iguanas (66 hatchlings, three subadults, seven adults), with hatchlings from nine sites (Gamboa, Panama and eight sites on or around Barro Colorado Island, Panama). We included an equal number of hatchlings from each site when possible. Hatchling social behavior (i.e., size of group, proximity to others) was observed haphazardly for individuals for about 60 days post-emergence over two years. (Adults and subadults were sampled and included whenever possible.) We primarily included samples taken about 4-6 weeks into the season for direct comparisons across sites and social levels. We have multiple samples (mean 3 per lizard) for 12 hatchlings spanning several days to several weeks between samples.

本数据集涵盖76只绿鬣蜥（Green Iguana）的后肠拭子样本，其中66只为幼体、3只为亚成体、7只为成体；幼体样本采自9个采样位点——巴拿马甘博阿（Gamboa）以及巴拿马巴罗科罗拉多岛及其周边的8个点位。若条件允许，我们从每个采样位点采集等量的幼体样本。研究人员在两年周期内，对所有幼体出壳后的约60天内，随机观察其社交行为，包括集群规模与个体间的空间接近程度。（成体与亚成体则在条件允许时均纳入采样范围。）本研究主要纳入采样季第4至6周采集的样本，以便在不同采样位点及不同社交层级间开展直接对照。针对12只幼体，我们采集了多份样本（单只个体平均采样3次），样本采集间隔介于数日至数周之间。