Time series of motor neuronal translatomes over the course of neuroinflammation

During the course of multiple sclerosis (MS), inflammatory insults drive neuro-axonal loss and disability progression. Here we leverage translating ribosome affinity purification (TRAP) to extract ribosome-bound mRNA from Chat-positive motor neurons of mice undergoing experimental autoimmune encephalomyelitis (EAE), the animal model of MS. This unique dataset allows to follow the temporal dynamics of neuronal responses to inflammation and enables the extractions of molecular targets for therapeutic intervention. Overall design: mRNA of Chat+ spinal cord motor neurons derived from mice undergoing experimental autoimmune encephalomyelitis (EAE) and healthy control mice was enriched by translating ribosome affinity purification (TRAP) and sequenced by RNA-seq.

在多发性硬化（multiple sclerosis, MS）的病程中，炎症损伤会驱动神经轴突丢失与残疾进展。本研究借助翻译核糖体亲和纯化（translating ribosome affinity purification, TRAP）技术，从罹患实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis, EAE，即多发性硬化的动物模型）的小鼠的Chat阳性运动神经元中提取结合于核糖体的mRNA。该独特数据集可追踪神经元对炎症应答的时间动态变化，并能够挖掘用于治疗干预的分子靶点。总体实验设计：通过翻译核糖体亲和纯化（TRAP）技术对来自EAE造模小鼠与健康对照小鼠的脊髓Chat+运动神经元的mRNA进行富集，随后采用RNA测序（RNA-seq）完成测序。