Analysis of non-coding RNAs in drn1∆ deletion strains

The turnover of introns spliced from pre-mRNA occurs first by debranching the lariat intron followed by destruction of the linear intron by other nucleases. We have identified a novel component of intron turnover, DRN1 (YGR093W). In order to identify RNAs affected by Drn1 turnover, we used a microarray containing probes specific to ~400 yeast non-coding RNAs to analyze a strain deleted for Drn1. These data implicated Drn1 in the turnover of a number of introns spliced from ribosomal-protein genes. Two-color experiment with biological replicate and dye swap.

从前体mRNA（pre-mRNA）上剪接产生的内含子周转过程，首先通过对套索状内含子实施脱分支反应启动，随后由其他核酸酶降解线性形式的内含子。本研究团队已鉴定出一种参与内含子周转的新型组分DRN1（YGR093W）。为了筛选受Drn1周转调控的RNA分子，我们使用了搭载有约400种酵母非编码RNA（non-coding RNAs）特异性探针的微阵列（microarray），对Drn1基因缺失菌株开展了分析。上述实验数据表明，Drn1参与了多种源自核糖体蛋白基因的内含子的周转过程。本研究采用了带有生物学重复与染料置换的双色实验方案。