Quantitative assessments of the anti-proliferative activity of tamoxifen (TMX) combinations with (A) NHAPI and Dp44mT and (B) their iron (Fe) and copper (Cu) complexes that were formed at an metal binding equivalent of 1.

All of the studied compounds and their combinations were incubated with MCF-7, T47D, or MDA-MB-231 cells for 72 h at 37°C at a concentration corresponding to their IC50 value. The combination index (CI) values were calculated using the Chou and Talalay method from n≥4 experiments using CalcuSyn 2.0 software; CI 1 indicate synergism, an additive effect, or antagonism, respectively. Statistical significance (ANOVA): *p0.05, **p0.01, ***p