Taxonomic evaluation of the genus Citrobacter using multilocus sequence analysis and description of Citrobacter pasteurii sp. nov.. Citrobacter pasteurii

Strains CIP 55.13T and CIP 55.9 formed a single distinct branch with C. youngae as the most closely related species, based on the phylogenetic analysis of sequences of genes fusA, pyrG, rpoB and leuS. Average nucleotide identity between CIP 55.13T and CIP 55.9 was 94.5% with Citrobacter youngae and Citrobacter freundii. Biochemical characteristics supported the fact that the two isolates represented a separate species. Based on phenotypic and genotypic results, strains CIP 55.13T and CIP 55.9 represent a novel species for which the name Citrobacter pasteurii sp. nov. is proposed; the type strain is CIP 55.13T

基于fusA、pyrG、rpoB及leuS基因序列的系统发育分析，菌株CIP 55.13^T与CIP 55.9共同构成一个独立的单系分支，其最密切的亲缘物种为青年柠檬酸杆菌（Citrobacter youngae）。CIP 55.13^T与CIP 55.9之间的平均核苷酸一致性为94.5%，且二者与青年柠檬酸杆菌、弗氏柠檬酸杆菌（Citrobacter freundii）的平均核苷酸一致性水平相近。生化特性证实这两株分离菌代表一个独立物种。结合表型与基因型分析结果，菌株CIP 55.13^T与CIP 55.9属于一个新物种，建议将其命名为巴斯德柠檬酸杆菌（Citrobacter pasteurii sp. nov.），其模式菌株为CIP 55.13^T