SSP-induced neurite-like process formation of SCLC cells: dependency on ECM components.

Cells were allowed to adhere to the particular substrates for 2 h and then subsequently treated with 50 nM SSP for 24 h. Values are given semiquantitatively: +: up to 25, ++: 26 to 50, +++: 51 to 75, ++++: 76 to 100% of process-bearing cells; -: no process formation; nc: no cell adhesion. *): Because H146 cells strongly tend to aggregate (see Fig. 2), the number of process-bearing cells could only roughly be estimated.将细胞接种于特定细胞培养底物上贴壁培养2小时，随后用50 nM的SSP处理24小时。结果以半定量方式标注："+"代表突起形成细胞占比≤25%，"++"代表26%~50%，"+++"代表51%~75%，"++++"代表76%~100%；"-"代表无突起形成；"nc"代表无细胞黏附。注*：由于H146细胞极易发生聚集（详见图2），因此仅能对带突起细胞的数量进行粗略估算。