Genome-wide measurement of spatial expression in mutants of Drosophila Melanogaster

We sequenced mRNA from transverse slices of embryos from a variety of D. melanogaster mutants (bicoid over-expression, bicoid knockdown, hunchback knocdown, and zelda mutant) at the blastoderm stage to determine genome-wide patterns of gene expression. Overall design: mRNA from transverse sections of single D. melanogaster embryos mutant for patterning TFs was sequenced.

本研究对处于囊胚期（blastoderm stage）的多种黑腹果蝇（Drosophila melanogaster，简称D. melanogaster）突变体胚胎的横切片进行mRNA测序，以解析全基因组范围的基因表达模式。所涉及的突变类型包括：bicoid过表达（bicoid over-expression）、bicoid基因敲低（bicoid knockdown）、hunchback基因敲低（hunchback knockdown，原文存在笔误knocdown）及zelda突变。整体实验设计：对携带模式形成相关转录因子（patterning Transcription Factor, TF）突变的单只黑腹果蝇胚胎的横切片的mRNA进行测序。