Evolutionary Conservation of PGBD3.

1We compiled CSB coding cDNA sequence by mapping the human cDNA to primate genomic sequences.
22 kb of intron sequence was analyzed beginning immediately upstream and downstream of the inverted repeats flanking PGBD3.
3The 3′ half of PGBD3P3 is inverted in human, chimp and Rhesus; conservation was calculated separately for the 5′ (+) and 3′ (−) portions of the pseudogenes.
4Orangutan genome assembly includes sequencing gaps that obscure 380 nt of the latter portion of CSB exon 18 and 55 nt of PGBD3; these regions were omitted from identity calculations.
n/p, Not present in draft genome assembly.

1. 我们通过将人类互补脱氧核糖核酸（complementary DNA，cDNA）比对至灵长类基因组序列，构建了CSB编码cDNA序列。
2. 针对PGBD3侧翼反向重复序列上下游紧邻区域内的2 kb内含子序列，我们开展了分析。
3. PGBD3P3的3'端半段在人类、黑猩猩与恒河猴中发生了倒位；我们针对该假基因的5'（+）与3'（-）区域分别计算了序列保守性。
4. 红毛猩猩基因组组装结果中存在测序间隙，导致CSB第18外显子后半段的380个核苷酸（nucleotide，nt）与PGBD3的55 nt区域无法被准确读取；此类区域未被纳入序列一致性计算。
n/p：未出现在基因组草图组装结果中。