Hippocampal tissue level case-control differential expression analysis (n=15 major depressive disorder cases versus n=15 unaffected matched controls)

A set of genes that were putatively differentially expressed in post mortem human hippocampal tissue (comparing n=15 cases and n=15 controls from the Stanley Neuropathology Consortium). RNAseq reads were aligned to reference index using kallisto (https://pachterlab.github.io/kallisto/about) and analysed for differential expression (https://pachterlab.github.io/sleuth/about). No transcripts were differentially expressed after Benjamini-Hochberg control for false discovery rate < 5%. So data shown are significant at nominal p<0.05 (FDR > 0.05, Benjamini-Hocherg control for false discovery rate)

本数据集收录了经推测存在差异表达的基因，样本来源为死后人类海马组织，对比组为来自斯坦利神经病理学联盟（Stanley Neuropathology Consortium）的15例病例样本与15例对照样本。本次研究使用kallisto工具（https://pachterlab.github.io/kallisto/about）将RNA测序（RNA-seq）读段比对至参考索引，并通过sleuth工具（https://pachterlab.github.io/sleuth/about）完成差异表达分析。经Benjamin-Hochberg错误发现率（false discovery rate, FDR）校正（阈值设定为<5%）后，未检测到存在显著差异表达的转录本。因此本次展示的数据为名义P值<0.05时的显著性结果（此时错误发现率FDR>5%，未通过Benjamin-Hochberg错误发现率校正）