Characterization of mouse monoclonal antibodies against GPCMV.

AELISA performed with soluble proteins; gB, gH/gL, gH/gL/gO and Pentamer = gH/gL/GP129/GP131/GP133. BExpression of gH/gL protein on the surface of 293T cells. CAntibody neutralizing potency as determined by the anti-gB immunofluorescence method. DSoluble gH/gL protein was used as immunogen.A. 采用可溶性蛋白开展酶联免疫吸附实验（Enzyme-Linked Immunosorbent Assay，ELISA），所用检测抗原包括gB、gH/gL、gH/gL/gO以及五聚体（Pentamer，即gH/gL/GP129/GP131/GP133）。 B. gH/gL蛋白在293T细胞表面的表达情况。 C. 采用抗gB免疫荧光法测定抗体的中和活性。 D. 以可溶性gH/gL蛋白作为免疫原。