RNAseq analysis of the N. punctiforme transcriptome during hormogonium development in wild type, and three sigma-factor deletion strains

Change in gene expression for wild-type Nostoc punctiforme ATCC 29133, or three derivative strains with sigma factors deleted, over the time course of hormogonium development Overall design: Total RNA from 3 biological replicates at 0,1,6,12, and 18 hours after hormogonium induction was ribodepleted, converted to cDNA, apended with adapters and sequenced in 6 lanes of a flow cell on an illumina HiSeq 4000 instrument野生型点状念珠藻（Nostoc punctiforme）ATCC 29133，或三株敲除σ因子（sigma factors）的衍生菌株，在藻殖段（hormogonium）发育全过程中的基因表达变化。整体实验设计：在藻殖段诱导后的0、1、6、12及18小时，分别收集3份生物学重复的总RNA，经核糖体RNA去除处理后反转录为cDNA，添加接头序列后，在Illumina HiSeq 4000测序仪的单个流通池（flow cell）的6个泳道中完成测序。