Pseudomonas sp. NLX4 Genome sequencing and assembly. Pseudomonas sp. NLX4

Relentless mining operations have destroyed our environment significantly. Soil inhabiting microbes play a significant role in ecological restoration of these areas. Microbial weathering processes like chemical dissolution of rocks significantly promotes the soil properties and enhances the rock to soil ratio respectively. Earlier studies have reported that bacteria exhibit efficient rock-dissolution abilities by releasing organic acids and other chemical elements from the silicate rocks. However, rock-dissolving mechanisms of the bacterium remain to be unclear till date. Thus, we have performed rock-dissolution experiments followed by genome and transcriptome sequencing of novel Pseudomonas sp.NLX-4 strain to explore the efficiency of microbe-mediated habitat restoration and its molecular mechanisms underlying this biological process. Results obtained from initial rock dissolution experiments revealed that Pseudomonas sp. NLX-4 strain efficiently accelerates the dissolution of silicate rocks by secreting amino acids, exopolysaccharides, and organic acids with elevated concentrations of potassium, silicon and aluminium elements. The rock dissolution experiments of NLX-4 strain exhibited an initial increase in particle diameter variation values between 0-15 days and decline after 15 days-time respectively.

无节制的采矿活动已对生态环境造成严重破坏。土壤栖居微生物在这类受损区域的生态修复中发挥关键作用。诸如岩石化学溶蚀的微生物风化过程，可显著改善土壤性状并提升岩土比。早期研究表明，细菌可通过从硅酸盐岩石中释放有机酸与其他化学元素，展现出高效的溶岩能力。然而，此类细菌的溶岩机制至今仍未阐明。因此，本研究针对新型假单胞菌（Pseudomonas）NLX-4菌株开展溶岩实验，并进行基因组与转录组测序，以探究微生物介导的生境修复效率及其背后的分子机制。初始溶岩实验结果显示，NLX-4菌株可通过分泌氨基酸、胞外多糖与有机酸，并提升钾、硅、铝元素的浓度，高效加速硅酸盐岩石的溶蚀过程。该菌株的溶岩实验还显示，其粒径变化值在0至15天内呈上升趋势，15天后则逐渐下降。