Analysis of Transcriptomics and Rhizosphere Fungal Diversity in Different Tissues of Blueberry under Cadmium Stress

In this study, blueberry transcriptomics and rhizosphere fungal diversity were analyzed by simulated potting method to treat blueberries with Cd stress, and the content of Fe, Mn, Cu, Zn and Cd in each tissue, soil and DGT of blueberries were determined. , Combined with transcriptomics for correlation analysis. A total of 84374 annotated genes were obtained in blueberry roots, stems, leaves and fruits, of which 3370 DEGs were found, and DEGs in the stem accounted for the highest proportion, totaling 2521. The annotation results show that these DEGs are mainly concentrated in a series of metabolic pathways related to signal transduction, defense and pathogenic response. Blueberries transfer excess Cd from the root to the stem for storage. The stem contains the highest Cd content, which is consistent with the transcriptomics analysis results, while the fruit contains the lowest Cd content. Correlation analysis between heavy metal content and transcriptomics results in each tissue was carried out, and a series of genes related to Cd regulation were screened. The blueberry root system relies on mycorrhiza to absorb nutrients in the soil. The intervention of Cd has severely affected the microflora structure of the blueberry rhizosphere soil. Coniochaetaceae, which is extremely tolerant, has gradually become the dominant population. Overall design: Apply CdSO4 in the form of an aqueous solution to control the Cd2+ concentration in the soil to 400.00mg/kg (calculated by the weight of pure metal). The control group is a potted plant (CK) without CdSO4, and the treatment is repeated three times for each group,Collect blueberry roots, branch, leaves and fruits for transcriptome sequencing

本研究采用模拟盆栽法对蓝莓施加镉（Cd）胁迫，开展蓝莓转录组学与根际真菌多样性分析，并测定了蓝莓各组织、土壤以及薄膜扩散梯度技术（DGT）中的铁（Fe）、锰（Mn）、铜（Cu）、锌（Zn）及镉（Cd）含量，结合转录组学数据开展相关性分析。
共从蓝莓根、茎、叶与果实中获得84374个注释基因，其中筛选得到3370个差异表达基因（Differentially Expressed Genes，DEGs）；茎部的差异表达基因占比最高，共计2521个。
注释结果显示，上述差异表达基因主要富集于信号转导、防御及病原响应相关的一系列代谢通路中。
蓝莓可将过量镉从根系转运至茎部进行储存，茎部镉含量最高，这与转录组学分析结果一致；而果实中的镉含量最低。
研究对各组织的重金属含量与转录组学结果开展相关性分析，筛选出一系列与镉调控相关的基因。
蓝莓根系依靠菌根吸收土壤中的养分，镉胁迫干预严重影响了蓝莓根际土壤的微生物群落结构，耐受性极强的炭团菌科（Coniochaetaceae）逐渐成为优势菌群。
试验设计方案：以水溶液形式施加硫酸镉（CdSO4），将土壤中镉离子（Cd²+）浓度控制为400.00mg/kg（以纯金属重量计）；对照组为未施加硫酸镉的盆栽植株（CK），每组均设置3次生物学重复，采集蓝莓根系、枝条、叶片及果实用于转录组测序。