The BbLar1-mediated transcriptome in Beauveria bassiana exposed to linoleic acid stress

Fungal tolerance is important for B. bassiana in biological control of insect pests. BbLar1 plays important roles in fungal resistance to linoleic acid stress. The genome-wide exprssion analysis involved in fungal resonse to unsaturated fatty acids was analyzed by using high throughput sequencing (RNA-Seq) at different concentrations of linoleic acid. Our analyese revealed that BbLar1 mediated numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in the tolerance of B. bassiana to linoleic acid stress. Comparison was conducted between the ΔBblar1 mutant strains at the indicated concentration of linoleic acid.

真菌耐受性在球孢白僵菌（Beauveria bassiana，简称B. bassiana）防治害虫的生物防治过程中具有重要意义。BbLar1在真菌抵抗亚油酸胁迫过程中发挥关键作用。本研究采用高通量测序技术（RNA-Seq），针对不同浓度亚油酸处理下的真菌不饱和脂肪酸应答反应开展全基因组表达分析。研究分析显示，BbLar1介导了大量差异表达基因（Differentially Expressed Genes，简称DEGs），这些基因涉及代谢、细胞转运与细胞应激救援过程，且显著参与球孢白僵菌对亚油酸胁迫的耐受调控。本研究在指定浓度的亚油酸处理条件下，对ΔBblar1突变菌株开展了对照比较分析。