Poly (A) Click-seq for U937 control and PMA-treated (6h and 24h) cells

Poly(A)-ClickSeq: click-chemistry for next-generation 3'-end sequencing without RNA enrichment or fragmentation enabling simultaneous genomewide poly(A)-site identification and differential expression analysis Overall design: poly(A)-directed RNA-seq experiments: Deep-sequencing for control vs 6h PMA-treated U937 cells, and control vs 24h PMA-treated U937 cells.聚腺苷酸点击测序（Poly(A)-ClickSeq）：一种无需RNA富集或片段化的下一代3'端测序点击化学技术，可同时实现全基因组范围内的聚腺苷酸化位点鉴定与差异表达分析。实验设计概览：聚腺苷酸靶向RNA测序实验，包含两组对比测序：对照组与经PMA处理6小时的U937细胞，对照组与经PMA处理24小时的U937细胞。