Imbalance of gut microbiota in gestational diabetes mellitus

Metagenomic sequencing and bioinformatics analyses were performed by Majorbio BioPharm Technology (Shanghai, China). Gene library was constructed using a TruSeqTM DNA Sample Prep Kit for Illumina. The amplified target DNA was sequenced by Majorbio on the Illumina MiSeq platform.Paired-end reads obtained by MiSeq sequencing were spliced according to overlap correlation, the sequence quality was controlled, and the sequences were filtered. After distinguishing the samples, operational taxonomic unit (OTU) cluster analysis and taxonomic analysis of species was performed. Based on the above statistical and visual analyses, such as multivariate analysis and significance test of difference, were performed on the flora composition and phylogenetic information of multiple samples.