Description of reference genes and primer sequences.

Primer PCR efficiency and PCR Tm product data represent mean values ± SE (n = 30). PCR efficiencies (E) calculated according to the equation (1 + E) = 10slope. Free energy for potential secondary structures in the amplicon is shown (ΔG, kcal/mole).引物PCR效率与PCR产物解链温度（PCR Tm）数据均以平均值±标准误（standard error，SE，n=30）的形式呈现。PCR效率（E）按照公式(1 + E) = 10^斜率 计算得出。扩增子（amplicon）潜在二级结构的自由能已给出（ΔG，单位：千卡/摩尔）。