GPER1 inhibits placental type I IFN responses to preserve fetal health during maternal infection [GECKOscreen]. GPER1 inhibits placental type I IFN responses to preserve fetal health during maternal infection [GECKOscreen]

Identification of factors that control ifn responses during pregnancy Overall design: A549 cells containing an interferon responsive reporter and CRISPR Cas9 technology were transduced with the complete sgRNA library and then treated with interferon alpha. Next, cells that were not responsive to interferon were collected and the sgRNAs enriched in these populations was sequenced.

妊娠期间干扰素应答调控因子的鉴定
整体实验设计：将搭载CRISPR-Cas9系统且携带干扰素应答报告基因的A549细胞，转导完整的单引导RNA（single guide RNA, sgRNA）文库，随后以α干扰素进行处理。后续收集对干扰素无应答的细胞，并对该细胞群体中富集的sgRNA进行测序。