Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide

Desmocollin-1 (DSC1) is a desmosomal transmembrane glycoprotein that maintains cell-to-cell adhesion. DSC1 was previously associated with lymph node metastasis of luminal A breast tumors and was found to increase metastatic potential of MCF7 cells in vitro. Therefore, we focused on DSC1 role in cellular and molecular mechanisms in luminal A breast cancer and its possible therapeutic modulation using parthenolide. We performed comparative gene expression analysis using data obtained from RNA-seq of DSC1-overexpressing and control cells treated and untreated with parthenolide in biological duplicates. Overall design: Comparative gene expression profiling analysis of RNA-seq data for MCF7-DSC1-GFP cells and control cell line MCF7-GFP treated and untreated with parthenolide.

桥粒胶蛋白-1（Desmocollin-1，DSC1）是一类维系细胞间黏附的桥粒跨膜糖蛋白。既往研究表明，DSC1与腔A型乳腺肿瘤的淋巴结转移存在关联，且体外实验证实其可增强MCF7细胞的转移潜能。据此，本研究聚焦于DSC1在腔A型乳腺癌中的细胞与分子机制的作用，以及采用小白菊内酯（parthenolide）对其进行治疗性调控的潜在可能。本研究开展比较基因表达分析，所用数据来源于经小白菊内酯处理与未处理的DSC1过表达细胞及对照细胞的生物学重复RNA测序（RNA-seq）样本。实验整体设计：对经小白菊内酯处理与未处理的MCF7-DSC1-绿色荧光蛋白（GFP）细胞及对照细胞系MCF7-GFP的RNA测序数据进行比较基因表达谱分析。