DNA methylation profiles of human primed and naive pluripotent stem cells

We introduce a method for generating transgene-free and high-quality naive human induced pluripotent stem cells (iPSCs) using a modified Sendai virus (SeV) vector reprogramming system. This reprogramming method realizes the derivation of naive iPSCs from various somatic cells accompanied with fast and robust SeV vector removal at early passages. The established naive iPSCs have superior naive-specific differentiation ability compared with iPSCs derived from conventional methods. Bisulphite converted DNA from the 13 samples were hybridised to the Illumina Infinium Human Methylation 450K or EPIC BeadChip Kit

本研究介绍了一种利用改造型仙台病毒（Sendai virus, SeV）载体重编程系统，制备无转基因且高质量初始态诱导多能干细胞（naive human induced pluripotent stem cells, iPSCs）的方法。该重编程方法可实现从多种体细胞中衍生初始态iPSCs，且在早期传代过程中即可快速且高效地清除SeV载体。所建立的初始态iPSCs相较于传统方法获得的iPSCs，具备更优异的初始态特异性分化能力。本研究将来自13份样本的亚硫酸氢盐转化DNA与Illumina Infinium人类甲基化450K或EPIC微珠芯片试剂盒进行了杂交。