Effect of LL-2 conditional medium on bone marrow-derived macrophages

To investigate the effect on LL-2 conditional medium on bone marrow-derived macrophages, equal volum of LL-2 conditional medium and fresh RPMI 1640 was used to culture bone marrow-derived macrophages for 24 hours. The cells were then lysed for RNA-seq. Overall design: Primary bone marrow-derived macrophages in 6 wells were divided into two group. Three wells were added with RPMI 1640 medium, while the other three wells were added with equal volum of LL-2 conditional medium and RPMI 1640 medium. Twenty four hours later, the cells were lysed for RNA-seq.

为探究LL-2条件培养基对骨髓来源巨噬细胞（bone marrow-derived macrophages）的影响，本实验采用等体积的LL-2条件培养基与新鲜RPMI 1640培养基共同培养骨髓来源巨噬细胞24小时，随后裂解细胞以进行RNA-seq测序。
实验整体设计：将6孔板中的原代骨髓来源巨噬细胞分为两组，其中3孔加入RPMI 1640培养基，剩余3孔加入等体积的LL-2条件培养基与RPMI 1640培养基。培养24小时后裂解细胞并开展RNA-seq测序。