RNAseq analysis of NR0B1 LOF

We report analysis of transcriptional chanages following RNAi depletion of NR0B1 or NRF2 depletion in NSCLC cell lines as determined by RNAseq. Additionally we also determine the genomic location of NR0B1 in NSCLC lines by Chip-Seq. Overall design: Evaulation of RNAseq following NR0B1 depeltion in one cell line and NRF2 depletion in two cell lines.

本研究报道了在非小细胞肺癌（Non-Small Cell Lung Cancer, NSCLC）细胞系中，经RNA干扰（RNA interference, RNAi）敲低NR0B1或NRF2后产生的转录组变化的分析结果，该分析通过RNA测序（RNA Sequencing, RNAseq）完成。此外，本研究还通过染色质免疫共沉淀测序（Chromatin Immunoprecipitation Sequencing, Chip-Seq）明确了NR0B1在非小细胞肺癌细胞系中的基因组定位。实验整体设计：在1株细胞系中开展NR0B1敲低后的RNA测序分析，并在2株细胞系中开展NRF2敲低后的RNA测序分析。