Crotalus simus Raw sequence reads

In this work we have obtained high quality whole transcriptome 454 reads (Titanium System, Roche Applied Science) from an adult and newborn individuals of Crotalus simus from Costa Rica as well as miRNA enriched libraries using Ion Torrent sequence technology (Life technologies, chip 314).A global characterization of the whole transcriptome reads was carried out according to the workflow we used in previous studies in order to find out whether the different proteome composition between individuals could be explained by different mRNA composition.

本研究获取了高质量的全转录组454测序读段（钛金测序系统，Roche Applied Science公司），样本来自哥斯达黎加的丛林响尾蛇（Crotalus simus）成体及新生个体；同时采用Ion Torrent测序技术（Life Technologies公司，314芯片）构建了富集微小RNA（miRNA）的文库。本研究依据既往研究中使用的分析流程，对全转录组测序读段开展全局特征分析，旨在探究个体间蛋白质组组成的差异是否可由信使RNA（mRNA）组成的差异加以解释。