Zinc-dependent regulation of zinc import and export genes by Zur. Streptomyces coelicolor

We determined the genomic locations of the Zur binding sites by chromatin immuno-precipitation of cross-linked DNA and hybridization on a tilling oligonucleotide array on exponentially grown cultures of M145 and zur mutant. Overall design: Three independent biological samples were prepared separately from the wild-type cells and pooled before hybridization on a single array. Three more independent biological samples were prepared from zur deletion mutant cells, pooled, and hybridized on another single array. After normalization, the signal from the mutant cells was subtracted from the signal from the wild-type cells to recover Zur specific signal.

本研究通过对交联DNA进行染色质免疫沉淀（chromatin immunoprecipitation），并在M145菌株与zur突变体的指数生长期培养物的平铺式寡核苷酸阵列上进行杂交实验，以此明确Zur结合位点的基因组定位。
整体实验设计如下：从野生型细胞中分别制备三份独立的生物学重复样本，混合后在单张芯片上完成杂交；另外从zur缺失突变体细胞中制备三份独立的生物学重复样本，混合后在另一张单芯片上进行杂交。
完成归一化处理后，将突变体细胞的信号值从野生型细胞的信号值中减去，以此获得Zur特异性结合信号。