Reprogram-Seq: A platform for single-cell combinatorial reprogramming [VI]. Reprogram-Seq: A platform for single-cell combinatorial reprogramming [VI]

Reprogram-Seq leverages organ-specific cell atlas data with single-cell perturbation and computational analysis to predict, evaluate, and optimize TF combinations that reprogram a cell type of interest. Overall design: Focusing on the cardiac system, we performed Reprogram-Seq on P0 mouse heart cells to generate a reference transcriptomic map. Based on the reference map, we selected TF candidates and tests 1000s of TF cocktails for direct lineage conversion by scRNA-Seq.

Reprogram-Seq 依托器官特异性细胞图谱数据、单细胞扰动实验与计算分析，可对目标细胞类型重编程所需的转录因子（Transcription Factor）组合进行预测、评估与优化。实验整体设计：本研究聚焦心脏系统，以出生后0天（P0）小鼠心肌细胞为样本开展Reprogram-Seq实验，构建参考转录组图谱。基于该参考图谱，我们筛选出候选转录因子，并通过单细胞RNA测序（single-cell RNA sequencing, scRNA-Seq）测试数千种转录因子组合，以实现直接谱系重编程。