Study of differential chromatin accessibility within primary breast epithelial cells from healthy donor and its TONSL overexpressing counterpart

To evaluate the probable role of TONSL during tumorigenesis we overexpressed TONSL in primary breast epithelial cells and studied differential chromatin accessibility. KTB103 primary and TONSL overexpressing KTB103 cells were subjected to ATAC-seq using the previously established protocol (Oncogene 40:1332-1346) . Assays were done in biologic triplicates with ~50,000 cells.

为探究TONSL在肿瘤发生过程中的潜在作用，我们在原代乳腺上皮细胞中过表达TONSL，并对差异染色质可及性进行分析。我们以KTB103原代细胞与过表达TONSL的KTB103细胞为研究材料，采用已发表的实验方案（Oncogene 40:1332-1346）开展转座酶可及性测序（ATAC-seq）。所有实验均设置3次生物学重复，每组细胞投入量约为50000个。