Single nuclei transcriptomic profiling of adult Drosophila upon oxidative stress

Environmental factors, infection, or injury, can cause oxidative stress in diverse tissues and loss of tissue homeostasis. Effective stress response cascades, conserved from invertebrates to mammals, ensure reestablishment of homeostasis and tissue repair. Hemocytes, the Drosophila blood-like cells, rapidly respond to oxidative stress by immune activation. However, the precise signals how they sense oxidative stress and integrate these signals to modulate their immune response during oxidative stress are ill-defined. Furthermore, it remains unclear if all hemocytes respond similarly to oxidative stress or if there is a specification in distinct subsets. here, we employes single nuclei RNA-sequencing (snRNA-seq) to elcuidate the induced transcriptomic chnages in hemocytes and other cell types by Paraquat feeding compared to control food. Paraquat induces a pronounced oxidative stress response in Drosophila and we used this mdoel to study the diversification of immune cells upon this immune challenge in the adult fly. Overall design: The data sets include two samples of sorted nuclei from flies on control food (100 flies/sample) and 2 samples on 15mM paraquat food (100 flies/sample)

环境因素、感染或损伤可诱发多种组织产生氧化应激，并造成组织稳态失衡。从无脊椎动物到哺乳动物均保守存在的有效应激应答通路，可保障机体重建稳态并完成组织修复。果蝇的类血细胞（hemocytes）可通过免疫激活快速响应氧化应激。然而，这类细胞感知氧化应激的精确信号机制，以及它们在氧化应激过程中整合信号以调控免疫应答的具体方式，仍未阐明。此外，目前尚不清楚所有血细胞是否对氧化应激具有一致的应答反应，或是不同血细胞亚群存在功能特化。
本研究采用单细胞核RNA测序（single nuclei RNA-sequencing，snRNA-seq）技术，对比分析百草枯（Paraquat）饲喂组与正常饲喂对照组中，血细胞及其他细胞类型的诱导转录组变化。百草枯可在果蝇体内引发显著的氧化应激应答，本研究借助该模型探究成年果蝇在该免疫刺激下免疫细胞的分化特征。
实验整体设计：本数据集包含2份来自正常饲喂果蝇的分选细胞核样本（每份样本含100只果蝇），以及2份来自15mM百草枯饲喂果蝇的分选细胞核样本（每份样本含100只果蝇）