Transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan

Serotonin is a monoamine that regulates processes such as energy balance and immune function. Manipulating this pathway in growing dairy calves could promote growth and development by modulating functions and signaling pathways within key organs. In this study, we characterized the adipose and muscle transcriptome of pre-weaned calves with increased serotonin bioavailability through the elucidation of differentially expressed genes. Overall design: Holstein bull calves (21d old) were fed milk replacer (8 L/d) supplemented with saline (CON, n=4) or 5-hydroxytryptophan (5HTP, n=4) for 10 d. Calves were euthanized on d 10 to harvest adipose and muscle tissue for RNA-Sequencing.

血清素（Serotonin）属于单胺类物质，可调控能量平衡、免疫功能等多种生理过程。在生长期乳用犊牛中干预该信号通路，或可通过调控关键器官的功能与信号转导通路，促进其生长发育。本研究通过解析差异表达基因，对血清素生物利用度提升的断奶前乳用犊牛的脂肪与肌肉转录组开展了特征分析。
总体实验设计：选取21日龄的荷斯坦（Holstein）公犊牛，每日饲喂8L代乳粉（milk replacer），分别添加生理盐水（对照组CON，n=4）或5-羟色氨酸（5-hydroxytryptophan，5HTP，n=4），持续饲喂10天。于第10日龄对犊牛实施安乐死，采集脂肪与肌肉组织用于RNA测序（RNA-Sequencing）。