Epicardial Differentiation Drives Fibro-fatty Remodeling in Arrhythmogenic Cardiomyopathy [scRNA-seq]

We performed single cell RNA sequencing (scRNAseq) on epicardial cells generated from human induced pluripotent stem cells (hiPSCs) of an arrhythmogenic cardiomyopathy patient and their PKP2 reverted isogenic control. The goal of this experiment was to analyze the process of epicardial to fibro-fatty celluar differentiation in diseased cells and compare the cellular transcirptomes of diseased and healthy cells. Overall design: scRNAseq of PKP2 mutant and reverted hiPSC-derived epicardial cells at day 70 of differentiation

本研究对致心律失常性心肌病（arrhythmogenic cardiomyopathy）患者的人类诱导多能干细胞（human induced pluripotent stem cells，hiPSCs）分化所得的心外膜细胞，及其经PKP2修复的同基因对照细胞，开展了单细胞RNA测序（single cell RNA sequencing，scRNAseq）。本实验的研究目标为分析病变细胞中心外膜向成脂纤维细胞分化的过程，并对比病变与健康细胞的细胞转录组。实验设计概述：对分化第70天的PKP2突变型及修复型hiPSC来源的心外膜细胞进行scRNAseq。