Study of microRNA associated with metformin’s anti-tumor effect [Huh7 cells]

The miRNAs expression was markedly altered with the treatment of metformin in vitro and in vivo and various miRNAs induced by metformin also may contribute to tumor growth in vitro and in vivo. Using a custom microarray platform, we analyzed the expression levels of 985 human miRNA probes in the cell lines in vitro and tumorous tissues in vivo that were treated with and without metformin.

经二甲双胍处理后，微小RNA（miRNAs）的表达在体外与体内模型中均发生显著改变；且二甲双胍诱导的多种微小RNA，亦可能在体外及体内环境中参与肿瘤生长过程。本研究采用定制化微阵列芯片平台，对分别经二甲双胍处理与未处理的体外细胞系及体内肿瘤组织中的985个人类微小RNA探针的表达水平进行了分析。