Bortezomib resistance in THP-1 cell line. Homo sapiens

Bortezomib is a proteasome inhibitor used in severel different hematological malignancies. Resistance to this drug is still poorly understood. In order get more insight in the resistance mechanism, we developed several bortezomib resistant subclones of the THP-1 monocytic/macrophage cell line. On these subclones expression arrays were performed. Overall design: We performed expression array three different bortezomib resistant subclones of the THP-1 cell line. The resistant subclones were spotted against the parental THP-1 wildtype cell line.

硼替佐米（Bortezomib）是一种蛋白酶体抑制剂，可用于多种血液系统恶性肿瘤的治疗。目前该药物的耐药机制仍不甚明确。为深入阐明其耐药机制，我们构建了多株THP-1单核细胞/巨噬细胞系的硼替佐米耐药亚克隆，并对这些亚克隆开展了基因表达微阵列（expression array）检测。整体实验设计：我们选取3株不同的THP-1细胞系硼替佐米耐药亚克隆进行基因表达微阵列分析，且以亲本THP-1野生型细胞系作为对照。