5eca0fc0-cd47-4103-8c55-307d130622e8 - samples

Tregs were sorted as CD4+CD25+CD127- cells from peripheral blood of 14 healthy individuals, 8 patients with mild/severe rheumatoid arthritis, 1 patient with systemic lupus erythematosus/rheumatoid arthritis, 2 patients with ulcerative colitis and 2 patients with Chrohn's disease. RNA was extracted and polyA libraries were prepared using the Illumina Truseq sample preparation kit v.2. Single-end 75bp sequencing was performed on NextSeq500.EGA dataset EGAD00001005446

本研究从14名健康个体、8名轻度/重度类风湿关节炎患者、1例合并系统性红斑狼疮与类风湿关节炎的患者、2名溃疡性结肠炎患者以及2名克罗恩病患者的外周血中，分选获得表型为CD4+CD25+CD127-的调节性T细胞（Tregs）。随后提取总RNA，采用Illumina Truseq样本制备试剂盒v.2构建polyA文库，并在NextSeq500测序平台上完成75bp单端测序。本数据集为EGA数据库收录的EGAD00001005446数据集。