Metabarcoding zooplankton at station ALOHA: Operational taxonomic unit (OTU) tables and fasta files for representative sequences from each OTU (Plankton Population Genetics project)

<p>This data submission consists of metabarcoding data for the zooplankton community in the epipelagic, mesopelagic and upper bathypelagic zones (0-1500m) of the North Pacific Subtropical Gyre. The goal of this study was to assess the hidden diversity present in zooplankton assemblages in midwaters, and detect vertical gradients in species richness, depth distributions, and community composition of the full zooplankton assemblage. Samples were collected in June 2014 from Station ALOHA (22.75, -158) using a 1-meter square&nbsp;Multiple Opening and Closing Nets and Environmental Sampling System (MOCNESS, 200um mesh), on R/V Falkor cruise FK140613. Next generation sequence data (Illumina MiSeq, V3 chemistry, 300-bp paired-end) of the zooplankton assemblage derive from amplicons of the V1-V2 region of 18S rRNA (primers described in Fonseca et al. 2010). The data includes sequences and read count abundance information for molecular OTUs from both holoplanktonic and meroplanktonic taxa.&nbsp;&nbsp;All results derive from analyses in&nbsp;mothur&nbsp;v1.36.1 (Schloss et al. 2009, Kozich et al. 2013).<strong> </strong></p> <p>Tables from four analyses are included in this submission:</p> <p>1) 97_OTUtable:&nbsp;read counts for each OTU (clustered at 97% similarity) across 54 samples (depth, size fractionated), with NCBI BLAST results for the top representative sequence from each OTU.&nbsp;</p> <p>2) 97_OTU_subsampled: read counts for each OTU (clustered at 97% similarity), subsampled for even sequencing coverage across 54 samples (depth, size fractionated, with NCBI BLAST results for the top representative sequence from each OTU.</p> <p>3) 99_OTU: read counts for each OTU (clustered at 99% similarity) across 54 samples (depth, size fractionated), with NCBI BLAST results for the top representative sequence from each OTU.&nbsp;</p> <p>4) 99_OTU_subsampled: read counts for each OTU (clustered at 99% similarity), subsampled for even sequencing coverage across 54 samples (depth, size fractionated), with NCBI BLAST results for the top representative sequence from each OTU.</p> <p>Related dataset containing NCBI accession numbers for sequence data:<br /> Metabarcoding zooplankton at station ALOHA: NCBI SRA accession numbers&nbsp;www.bco-dmo.org/dataset/700961</p>本数据集提交内容为北太平洋副热带环流（North Pacific Subtropical Gyre）海域真光层（epipelagic）、中层海（mesopelagic）及上层深海带（upper bathypelagic，0-1500米水深）的浮游动物群落元条形码（metabarcoding）数据。本研究旨在评估中层水域浮游动物群落中潜藏的物种多样性，并解析完整浮游动物群落在物种丰富度、深度分布及群落组成上的垂直梯度变化。样品于2014年6月采集自ALOHA站（坐标22.75, -158），使用1平方米多开口闭合网与环境采样系统（Multiple Opening and Closing Nets and Environmental Sampling System, MOCNESS，网孔孔径200μm），搭载于Falkor号科考船FK140613航次。浮游动物群落的下一代测序数据采用Illumina MiSeq平台，搭配V3化学试剂，进行300bp双端测序，其扩增子来源于18S rRNA基因的V1-V2高变区（引物信息参见Fonseca等人2010年发表的研究）。本数据集涵盖浮游终生生物（holoplanktonic）与阶段性浮游生物（meroplanktonic）类群的分子操作分类单元（operational taxonomic unit, OTU）序列及其读长计数丰度信息。所有分析结果均基于mothur v1.36.1软件生成（方法详见Schloss等人2009年、Kozich等人2013年发表的研究）。 本提交包含4项分析所得的表格： 1) 97_OTUtable：包含54个按深度、粒径分馏的样品中，每个以97%相似度聚类的OTU的读长计数，并附每个OTU的代表性序列的NCBI BLAST比对结果。 2) 97_OTU_subsampled：包含每个以97%相似度聚类的OTU的读长计数，已进行抽平处理以确保54个按深度、粒径分馏的样品间测序覆盖度一致，同时附每个OTU的代表性序列的NCBI BLAST比对结果。 3) 99_OTU：包含54个按深度、粒径分馏的样品中，每个以99%相似度聚类的OTU的读长计数，并附每个OTU的代表性序列的NCBI BLAST比对结果。 4) 99_OTU_subsampled：包含每个以99%相似度聚类的OTU的读长计数，已进行抽平处理以确保54个按深度、粒径分馏的样品间测序覆盖度一致，同时附每个OTU的代表性序列的NCBI BLAST比对结果。 本数据集包含序列数据的NCBI登录号相关信息：北太平洋ALOHA站浮游动物元条形码数据：NCBI SRA登录号详见www.bco-dmo.org/dataset/700961。