Summary of the conditions and results of each round of mutagenesis.

*Note that in the 3rd round of mutagenesis there was a technical problem with the temperation, and cultures were also temperated during the night cycle, leading to many more cells dying than usual. A recovery at lower temperature was used to rescue surviving cells.

*注：第三轮诱变处理过程中出现温度调控技术故障，且培养物在夜间培养周期内的温度调控也存在异常，导致细胞死亡量远高于常规水平。后续通过低温恢复培养的方式对存活细胞进行了挽救。