Whole transcriptome sequencing of lung tissue from septic mice

Acute lung injury (ALI) is a common complication of sepsis, which can progress to acute respiratory failure without timely intervention and affect patient prognosis. The underlying molecular mechanism of sepsis-mediated ALI is unclear. In the present study, we performed whole-transcriptome sequencing and identified a lncRNA/circRNA-miRNA-mRNA ceRNA network and signaling pathways to gain insight into the pathogenesis of ALI..

急性肺损伤（Acute lung injury, ALI）是脓毒症的常见并发症，若未及时干预可进展为急性呼吸衰竭，进而影响患者预后。脓毒症介导的ALI潜在分子机制目前尚不明确。本研究通过全转录组测序，鉴定得到长链非编码RNA（long non-coding RNA, lncRNA）/环状RNA（circular RNA, circRNA）-微小RNA（microRNA, miRNA）-信使RNA（messenger RNA, mRNA）内源竞争RNA（competing endogenous RNA, ceRNA）调控网络及相关信号通路，以期深入解析ALI的发病机制。