Ailanthus excelsa wood transctiptome

Ailanthus excelsa Roxb. belonging to family Simaroubaceae is a multi-purpose tree species and the wood is extensively used for production of match splints. The wood transcriptome of a 22 months old tree was sequenced in Illumina HiSeq 2500 using 150 bp paired-end chemistry. De novo assembly predicted a total of 1,10,604 transcripts which clustered into 48,493 unigenes with N50 of 1,606 bp. Functional annotation, pathway analysis, transcription factor and microsatellite predictions were conducted using different computational pipelines. This is the first wood genomic resource in the genus Ailanthus and the information generated can be used for accelerated breeding in the species.

隶属于苦木科（Simaroubaceae）的大果臭椿（Ailanthus excelsa Roxb.）是一种多用途树种，其木材被广泛用于制作火柴梗。以一株22月龄植株的木材为实验材料，采用150 bp双端测序化学方案，在Illumina HiSeq 2500平台上完成转录组测序。经从头组装，共预测得到110604条转录本，这些转录本聚类为48493条非冗余基因（unigene），其N50值为1606 bp。依托不同生物信息学分析流程，完成了功能注释、通路分析、转录因子预测以及微卫星标记预测等相关分析。本资源为臭椿属（Ailanthus）首个木材组学相关基因组资源，所生成的研究数据可用于该物种的加速育种工作。