CIL:13377

z-focal series through Hela cell in metaphase illustrating MCAK localization in Bod-1siRNA cells. Cells were transfected with control (this image) or Bod1 (CIL: 13378) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing. Cells were stained for total mitotic centromere-associated kinesin (MCAK) population. MCAK localizes to the inner centromere in its phosphorylated form but concentrates at kinetochores in its dephosphorylated state.本数据集包含中期海拉细胞的Z轴聚焦序列图像，用于展示有丝分裂着丝粒相关驱动蛋白（Mitotic Centromere-Associated Kinesin，MCAK）在Bod-1小干扰RNA（small interfering RNA，siRNA）处理细胞中的定位情况。实验中细胞分别转染了对照小干扰RNA（本图像即对应此对照组）或Bod-1（CIL: 13378）小干扰RNA。转染72小时后，用单斯特罗尔（monastrol）处理细胞3小时，随后将细胞转移至含有蛋白酶体抑制剂MG132的培养基中孵育1小时，之后进行细胞固定。通过免疫荧光染色标记总MCAK蛋白。MCAK以磷酸化形式定位于内着丝粒，而去磷酸化状态下则富集于动粒。