A P. aeruginosa serotype-defining single read from our first Oxford Nanopore run

Here is, I think, the first publically-available Oxford Nanopore read to be published. This came off our MinION instrument this morning (Wednesday 11th June). The DNA was derived from Pseudomonas aeruginosa strain 910 which originally came from hospital water. DNA was fragmented with Covaris G-Tube as per the Oxford Nanopore standard genomic library preparation protocol. The read maps to part of the P. aeruginosa O6-antigen determining region.笔者认为，本文所发布的为首款可公开获取的牛津纳米孔（Oxford Nanopore）测序读长序列。该序列于6月11日周三当日上午由本实验室的MinION测序仪产出。实验所用DNA来源于铜绿假单胞菌（Pseudomonas aeruginosa）菌株910，该菌株最初分离自医院水环境。实验按照牛津纳米孔标准基因组文库制备流程，使用Covaris G-Tube对DNA进行片段化处理。该测序读长比对至铜绿假单胞菌O6抗原决定区域的部分序列。