Enhanced efficacy of simultaneous PD-1 and PD-L1 immune checkpoint blockade in high grade serous ovarian cancer [bulk RNA-seq tumor]. Enhanced efficacy of simultaneous PD-1 and PD-L1 immune checkpoint blockade in high grade serous ovarian cancer [bulk RNA-seq tumor]

Immune therapies have had limited efficacy in high grade serous ovarian cancer (HGSC). The cellular targets and mechanism(s) of action of these agents in HGSC are unknown. Here we performed immune functional and single cell RNA sequencing transcriptional profiling on novel HGSC organoid/immune cell co-cultures treated with a variety of immune checkpoint blockade (ICB) antibodies compared to controls. Overall design: Bulk RNA seq on 13 tumor samples >>>Raw data not available due to patient privacy concerns<<<

免疫治疗在高级别浆液性卵巢癌（high grade serous ovarian cancer, HGSC）中的疗效有限。此类治疗药物在高级别浆液性卵巢癌中的细胞靶点与作用机制仍不明确。本研究针对经多种免疫检查点阻断（immune checkpoint blockade, ICB）抗体处理的新型高级别浆液性卵巢癌类器官-免疫细胞共培养体系，开展了免疫功能检测与单细胞RNA测序转录组分析，并设置对照组进行对比。整体实验设计：对13份肿瘤样本进行批量RNA测序>>>因患者隐私保护问题，原始数据暂不公开<<<