Transcriptional profiles of Th cells induced to polarize to Th1 or Th2 direction in the presence or absence of TGFbeta. Homo sapiens

Th1 and Th2 cells arise from a common precursor cell in response to triggering through the TCR and cytokine receptors for IL-12 or IL-4. This leads to activation of complex signaling pathways, which are not known in detail. Disturbances in the balance between type 1 and type 2 responses can lead to certain immune-mediated diseases. Thus, it is important to understand how Th1 and Th2 cells are generated. To clarify the mechanisms as to how IL-12 and IL-4 induce Th1 and Th2 differentiation and how TGF-beta can inhibit this process, we have used oligonucleotide arrays to examine the early polarization of Th1 and Th2 cells in the presence and absence of TGF-beta after 0, 2, 6 and 48 hours of polarization. Keywords: time course, differentiation Overall design: This study includes altoghether 34 samples. Six different types of treatments (Thp, Th0, Th1, Th2, Th1+TGFbeta, Th2+TGFbeta) were studied at 4 time points (0, 2, 6 and 48h). There are two biological replicates, for both time series, consisting of pooled samples derived from different individuals. The early time points (0, 2 and 6h) and late time point (0 and 48h) were done in separate experiments (cell from different individuals), because of the limited number of the cells obtained from each the cord blood.

Th1与Th2细胞均源自共同的前体细胞，在T细胞受体（TCR）以及IL-12或IL-4的细胞因子受体介导的触发信号刺激下产生。该过程会激活复杂的信号通路，但其具体调控机制尚未完全明确。1型与2型免疫应答的平衡紊乱可引发多种免疫介导性疾病，因此阐明Th1与Th2细胞的生成调控机制具有重要的科学意义。为阐明IL-12、IL-4诱导Th1与Th2细胞分化的具体机制，以及转化生长因子β（TGF-β）抑制该分化过程的原理，本研究采用寡核苷酸芯片（oligonucleotide array），检测了在Th细胞极化诱导0、2、6和48小时后，存在或不存在TGF-β时Th1与Th2细胞的早期极化状态。
关键词：时间进程，细胞分化
实验设计概述：本研究共包含34份样本。本研究设置了6种不同的处理组（Thp、Th0、Th1、Th2、Th1+TGF-β、Th2+TGF-β），并在4个时间点（0、2、6和48小时）对各组进行检测。两类时间序列实验均设置2次生物学重复，实验样本为混合自不同个体的细胞样本。由于每份脐血获取的细胞数量有限，早期时间点（0、2和6小时）与晚期时间点（0和48小时）的实验分别使用了不同个体来源的脐血细胞完成。