Additional file 2 of PRV-1b and PRV-3a infection is associated with the same clinical disease in coho salmon (Oncorhynchus kisutch) farmed in Chile: unraveling the pathogenesis of the orthoreoviral cardiomyopathy and hemolytic jaundice (OCHJ)

Additional file 2. Histoscore Liver (hsLiver). Histopathological criteria and semi-quantitative weighting used to define histoscore in the liver (hsLiver). Abbreviations: NHC: no histological changes; NIC: no inflammation changes; HCV: hepatocellular cytoplasm vacuoles; TS: tissue surface; NII: necrosis or inflamatory infiltrate; MMC: melanomacrophage cells; HV: hepatocellular vacuolization; NCR: nucleus:cytoplasm ratio; HPZ: hepatic portal zone. Interpretation: hsLiver ≤ 0.9 means a mild liver damage; hsLiver > 0.9 but ≤ 1.8, means a moderate damage; and hsLiver > 1.8 means a severe damage.

补充材料2：肝脏组织评分（Histoscore Liver，简称hsLiver）。用于定义肝脏组织评分（hsLiver）的组织病理学判定标准与半定量加权规则。
缩写说明：NHC：无组织学改变（no histological changes）；NIC：无炎症改变（no inflammation changes）；HCV：肝细胞胞质空泡（hepatocellular cytoplasm vacuoles）；TS：组织表面（tissue surface）；NII：坏死或炎性浸润（necrosis or inflammatory infiltrate，原文存在拼写笔误inflamatory）；MMC：黑素巨噬细胞（melanomacrophage cells）；HV：肝细胞空泡变性（hepatocellular vacuolization）；NCR：核质比（nucleus:cytoplasm ratio）；HPZ：肝门区（hepatic portal zone）。
判读标准：hsLiver≤0.9提示轻度肝损伤；hsLiver＞0.9且≤1.8提示中度肝损伤；hsLiver＞1.8提示重度肝损伤。