Single-cell transcriptional profiling of cancer cells in hairy cell leukemia (HCL) patients

This single-cell RNA sequencing (scRNA-seq) dataset profiles human HCL cells from two patients with Hairy Cell Leukemia (HCL) and varient subtype of HCL (HCLv), both expressing unmutated IGHV4-34. scRNA-seq was performed on isolated CD19? and CD3?cells from peripheral blood mononuclear cells (PBMC) (n=2). The dataset enables the characterization of expressed mutation and transcriptional profiles of human HCL cells and supports investigations into cellular lineage and pathways associated with pathologic activation leading to HCL and variant form of HCL (HCLv). Overall design: This study (protocol# 10C0066) was approved by the National Institutes of Health Institutional Review Board (IRB). Fresh peripheral blood were collected from 2 patients diagnosed with HCL undergoing standard-of-care therapeutic treatment. All patients signed IRB-approved informed consent permitting the use of their blood for research. PBMCs were purified from peripheral blood, and cells positive for either CD19 or CD3 were isolated for full-length single-cell sequencing. The expressed mutations and transcriptional profiles were analyzed for identifying the lienage and developmental state of the single cells.

本单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）数据集对2例毛细胞白血病（Hairy Cell Leukemia, HCL）及毛细胞白血病变异亚型（HCLv）患者的人HCL细胞进行了表征，二者均表达未突变的IGHV4-34基因。研究人员从外周血单个核细胞（peripheral blood mononuclear cells, PBMC）中分离得到CD19阳性及CD3阳性细胞，并对其开展了scRNA-seq实验（样本量n=2）。该数据集可用于解析人HCL细胞的表达突变特征与转录组谱，并为探究与HCL及其变异型（HCLv）病理激活相关的细胞谱系及通路提供研究支持。实验设计：本研究（实验方案编号：10C0066）已获得美国国立卫生研究院机构审查委员会（National Institutes of Health Institutional Review Board, IRB）批准。研究人员从2例确诊为HCL且接受标准治疗的患者体内采集新鲜外周血，所有患者均签署了经IRB批准的知情同意书，同意将其血液用于科研用途。研究人员从外周血中纯化得到外周血单个核细胞，随后分离CD19阳性或CD3阳性细胞用于全长单细胞测序。通过分析表达突变与转录组谱，本研究旨在鉴定单细胞的谱系及发育状态。